MAGeCK CRISPR Analyzer Skill

Purpose

Provide MAGeCK CRISPR screen analysis for gene essentiality scoring.

Capabilities

• Guide read counting
• Normalization and QC
• Gene-level analysis
• Pathway enrichment
• MLE model for complex designs
• Visualization outputs

Usage Guidelines

• Count guides accurately from sequencing data
• Normalize counts appropriately
• Calculate gene-level scores
• Enrich for biological pathways
• Use MLE for complex experimental designs
• Generate informative visualizations

Dependencies

• MAGeCK
• BAGEL
• CRISPResso2

Process Integration

• CRISPR Screen Analysis (crispr-screen-analysis)